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What is the basic definition of homeostasis?
Homeostasis is the maintenance of stable internal conditions (temperature, pH, osmotic balance) within an organism despite external environmental changes, achieved through negative feedback mechanisms.
Explain the relationship between genotype and phenotype.
Genotype is the organism's genetic makeup (alleles present); phenotype is the observable physical or biochemical characteristic. Phenotype results from genotype + environmental factors: Phenotype = Genotype + Environment.
What is the role of ATP in cells?
ATP (adenosine triphosphate) is the primary energy currency of cells. When the phosphate bond breaks (ATP → ADP + Pi), energy is released and used to power active transport, muscle contraction, protein synthesis, and other cellular work.
How does negative feedback maintain homeostasis? Provide an example.
Negative feedback detects deviation from a set point, then triggers a response that opposes the change and returns the system to normal. Example: High blood glucose → pancreas releases insulin → glucose taken up by cells → blood glucose decreases back to normal.
What is the difference between mitosis and meiosis in terms of chromosome number and daughter cells?
Mitosis produces 2 identical diploid daughter cells (2n → 2n); used for growth and repair. Meiosis produces 4 unique haploid daughter cells (2n → n); used for sexual reproduction. Meiosis includes two divisions; mitosis is one.
Describe the mechanism of passive transport and give one example.
Passive transport moves substances across a membrane without energy (ATP), along the concentration gradient from high to low concentration. Example: Oxygen diffusion into cells or water movement via osmosis through aquaporins.
What is enzyme catalysis and why do enzymes lower activation energy?
Enzyme catalysis is acceleration of a chemical reaction by an enzyme (protein catalyst). Enzymes lower activation energy by stabilizing the transition state, allowing reactions to proceed at body temperature without heat. This increases reaction rate without being consumed.
How does photosynthesis convert light energy into chemical energy? What are the two main stages?
Photosynthesis captures light energy in chlorophyll and converts it to chemical energy in glucose. Light-dependent reactions (thylakoids): light → ATP + NADPH + O₂. Light-independent reactions/Calvin cycle (stroma): ATP + NADPH → glucose from CO₂.
Explain how natural selection works using the reasoning: variation → heredity → differential survival.
Genetic variation in populations allows individuals to differ in traits. Heritable traits are passed to offspring. Individuals with advantageous traits survive/reproduce better (differential survival/reproduction), increasing those allele frequencies in the population over generations.
What is the central dogma of molecular biology and how does it explain protein synthesis?
Central dogma: DNA → RNA → Protein. DNA is transcribed to mRNA in the nucleus. mRNA is translated at ribosomes into proteins using tRNA to match codons with amino acids. This explains how genetic information controls organism traits and function.
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